abstract：:Ene-reductases originating from extremophiles are gaining importance in the field of biocatalysis due to higher-stability properties. The genome of the acidophilic iron-oxidizing bacterium "Ferrovum" sp. JA12 was found to harbor a thermophilic-like ene-reductase (FOYE-1). The foye-1 gene was ligated into a pET16bp exp...

journal_title：Applied microbiology and biotechnology

authors： Scholtissek A,Ullrich SR,Mühling M,Schlömann M,Paul CE,Tischler D

更新日期：2017-01-01 00:00:00

abstract：:Five yeast strains, Saccharomyces cerevisiae D8, M12, and S13; Hanseniaspora uvarum S6; and Issatchenkia orientalis KMBL5774, isolated from Korean grapes, were entrapped in Ca-alginate beads, which are non-toxic, simple to use, and economical. Ca-alginate beads containing yeast cells were soaked in protective solution...

journal_title：Applied microbiology and biotechnology

authors： Kim DH,Lee SB,Park HD

更新日期：2017-01-01 00:00:00

abstract：:MeBglD2, a β-glycosidase that is highly activated in the presence of various monosaccharides and disaccharides, was isolated from a soil metagenomic library. MeBglD2 had not only β-glucosidase activity but also β-galactosidase and β-fucosidase activities. MeBglD2 β-glucosidase activity increased in a cellobiose concen...

journal_title：Applied microbiology and biotechnology

authors： Matsuzawa T,Yaoi K

更新日期：2017-01-01 00:00:00

abstract：:Terminal disinfection and daily cleaning have been performed in hospitals in Taiwan for many years to reduce the risks of healthcare-associated infections. However, the effectiveness of these cleaning approaches and dynamic changes of surface microbiota upon cleaning remain unclear. Here, we report the surface changes...

journal_title：Applied microbiology and biotechnology

authors： Chen CH,Tu CC,Kuo HY,Zeng RF,Yu CS,Lu HH,Liou ML

更新日期：2017-01-01 00:00:00

abstract：:A bench-scale granular autotrophic nitrogen removal bioreactor (completely autotrophic nitrogen removal over nitrite (CANON) system) used for the treatment of synthetic wastewater was analyzed for the identification of microbiota with potential capacity for carbonate and phosphate biomineral formation. 16S ribosomal R...

journal_title：Applied microbiology and biotechnology

authors： Gonzalez-Martinez A,Rodriguez-Sanchez A,Rivadeneyra MA,Rivadeneyra A,Martin-Ramos D,Vahala R,Gonzalez-Lopez J

更新日期：2017-01-01 00:00:00

abstract：:A novel and efficient screening method for pyrroloquinoline quinone (PQQ) high-yielding methylotrophic strains was developed by using glucose dehydrogenase apoenzyme (GDHA) which depended on PQQ as the cofactor. Using this high-throughput method, PQQ high-yielding strains were rapidly screened out from thousands of me...

journal_title：Applied microbiology and biotechnology

authors： Si Z,Zhu J,Wang W,Huang L,Wei P,Cai J,Xu Z

更新日期：2016-12-01 00:00:00

abstract：:Rhamnolipids produced by Pseudomonas aeruginosa are biosurfactants with a high biotechnological potential, but their extensive commercialization is limited by the potential virulence of P. aeruginosa and by restrictions in producing these surfactants in heterologous hosts. In this work, we report the characterization ...

journal_title：Applied microbiology and biotechnology

authors： Grosso-Becerra MV,González-Valdez A,Granados-Martínez MJ,Morales E,Servín-González L,Méndez JL,Delgado G,Morales-Espinosa R,Ponce-Soto GY,Cocotl-Yañez M,Soberón-Chávez G

更新日期：2016-12-01 00:00:00

abstract：:Cyanobacterial mutants defective in acyl-acyl carrier protein synthetase (Aas) secrete free fatty acids (FFAs) into the external medium and hence have been used for the studies aimed at photosynthetic production of biofuels. While the wild-type strain of Synechocystis sp. PCC 6803 is highly sensitive to exogenously ad...

journal_title：Applied microbiology and biotechnology

authors： Kojima K,Keta S,Uesaka K,Kato A,Takatani N,Ihara K,Omata T,Aichi M

更新日期：2016-12-01 00:00:00

abstract：:Expressing proteins with fusion partners improves yield and simplifies the purification process. We developed a novel fusion partner to improve the secretion of heterologous proteins that are otherwise poorly excreted in yeast. The VOA1 (YGR106C) gene of Saccharomyces cerevisiae encodes a subunit of vacuolar ATPase. W...

journal_title：Applied microbiology and biotechnology

authors： Bae JH,Sung BH,Seo JW,Kim CH,Sohn JH

更新日期：2016-12-01 00:00:00

abstract：:Bacterial glucans have aroused increasing interest in commercial applications in the food and pharmaceutical sectors. A number of bacterial glucans have been reported over recent decades, and their structure, production, and functional properties have been extensively studied. In this paper, we review recent researche...

journal_title：Applied microbiology and biotechnology

authors： Xu L,Zhang J

更新日期：2016-11-01 00:00:00

abstract：:Clostridium acetobutylicum is an important industrial microorganism for n-butanol bioproduction, and its transcription factor AbrB0310 regulates various important cellular processes. However, the roles of two abrB homologues, abrB1941 and abrB3647, have not been determined because they appear inactive during transcrip...

journal_title：Applied microbiology and biotechnology

authors： Xue Q,Yang Y,Chen J,Chen L,Yang S,Jiang W,Gu Y

更新日期：2016-11-01 00:00:00

abstract：:Mitigation of the methane (CH4) emission from ruminants is needed to decrease the environmental impact of ruminant animal production. Different plant materials and chemicals have been tested, but few are both effective and practical. Medicinal herbs contain biological compounds and antimicrobials that may be effective...

journal_title：Applied microbiology and biotechnology

authors： Wang J,Liu M,Wu Y,Wang L,Liu J,Jiang L,Yu Z

更新日期：2016-11-01 00:00:00

abstract：:Flow cytometry is a rapid and quantitative method to determine bacterial viability. Although different stains can be used to establish viability, staining protocols are inconsistent and lack a general optimization approach. Very few "true" multicolor protocols, where dyes are combined in one sample, have been develope...

journal_title：Applied microbiology and biotechnology

authors： Buysschaert B,Byloos B,Leys N,Van Houdt R,Boon N

更新日期：2016-11-01 00:00:00

abstract：:Increased pentose phosphate pathway flux, relative to total substrate uptake flux, is shown to enhance succinic acid (SA) yields under continuous, non-growth conditions of Actinobacillus succinogenes biofilms. Separate fermentations of glucose and xylose were conducted in a custom, continuous biofilm reactor at four d...

journal_title：Applied microbiology and biotechnology

authors： Bradfield MF,Nicol W

更新日期：2016-11-01 00:00:00

abstract：:The aim of this study was to determine the antimicrobial effects of lemongrass essential oil (C. flexuosus) and to determine cytotoxic effects of both test compounds on human dermal fibroblasts. Antimicrobial susceptibility screening was carried out using the disk diffusion method. Antimicrobial resistance was observe...

journal_title：Applied microbiology and biotechnology

authors： Adukwu EC,Bowles M,Edwards-Jones V,Bone H

更新日期：2016-11-01 00:00:00

abstract：:Microbial strains of the genera Dietzia, Micrococcus, Pseudomonas, Rhodococcus, Gordonia, Streptomyces, Pseudomonas, Bacillus, Penicillium, Rhodotorula and Lactobacillus were screened for the ability to convert chalcones. Synthesis of chalcones was performed by the Claisen-Schmidt reaction. There were three groups of ...

journal_title：Applied microbiology and biotechnology

authors： Stompor M,Kałużny M,Żarowska B

更新日期：2016-10-01 00:00:00

abstract：:Studies have shown that external stress induces biofilm formation, but the underlying details are not clearly understood. This study investigates the changes in cell surface properties leading to increase in biofilm formation by Staphylococcus aureus and Pseudomonas aeruginosa in the presence of streptomycin. Bacteria...

journal_title：Applied microbiology and biotechnology

authors： Kumar A,Ting YP

更新日期：2016-10-01 00:00:00

abstract：:Multidrug-resistant Acinetobacter baumannii is a well-documented pathogen associated with hospital-acquired infections. In addition to multidrug resistance, A. baumannii can also become resistant to colistin, the antibiotic treatment of last resort, by the loss of the lipopolysaccharide from its outer membrane. Here, ...

journal_title：Applied microbiology and biotechnology

authors： Tseng CC,Tsai YH,Hu A,Liou JW,Chang KC,Chang HH

更新日期：2016-10-01 00:00:00

abstract：:The accumulation of toxic inorganic nitrogen is one of the major water quality problems in intensive aquaculture systems, thus the N removal in aquaculture systems is an important issue for the sustainable development of aquaculture. To understand one of the major microbial N removal processes, anaerobic ammonium oxid...

journal_title：Applied microbiology and biotechnology

authors： Li M,Gu JD

更新日期：2016-10-01 00:00:00

abstract：:The bean bug, Riptortus pedestris, is a major agricultural pest that reduces crop quality and value. Chemical pesticides have contributed to pest management, but resistance to these chemicals has significantly limited their use. Alternative strategies with different modes of action, such as entomopathogenic fungi, are...

journal_title：Applied microbiology and biotechnology

authors： Kim S,Lee SJ,Nai YS,Yu JS,Lee MR,Yang YT,Kim JS

更新日期：2016-10-01 00:00:00

abstract：:The Gram-positive Corynebacterium glutamicum is widely used for fermentative production of amino acids. The world production of L-lysine has surpassed 2 million tons per year. Glucose uptake and phosphorylation by C. glutamicum mainly occur by the phosphotransferase system (PTS) and to lesser extent by inositol permea...

journal_title：Applied microbiology and biotechnology

authors： Pérez-García F,Peters-Wendisch P,Wendisch VF

更新日期：2016-09-01 00:00:00

abstract：:His-Asn-His (HNH) proteins are a very common family of small nucleic acid-binding proteins that are generally associated with endonuclease activity and are found in all kingdoms of life. Although HNH endonucleases from mesophiles have been widely investigated, the biochemical functions of HNH endonucleases from thermo...

journal_title：Applied microbiology and biotechnology

authors： Zhang L,Huang Y,Xu D,Yang L,Qian K,Chang G,Gong Y,Zhou X,Ma K

更新日期：2016-09-01 00:00:00

abstract：:Antimicrobial peptides/proteins (AMPs) are biologically active molecules with diverse structural properties that are produced by mammals, plants, insects, ticks, and microorganisms. They have a range of antibacterial, antifungal, antiviral, and even anticancer activities, and their biological properties could therefor...

journal_title：Applied microbiology and biotechnology

authors： Tonk M,Vilcinskas A,Rahnamaeian M

更新日期：2016-09-01 00:00:00

abstract：:Biofilms are widely used in wastewater treatment for their particular enhancement of nitrogen removal and other significant advantages. In this study, the diversity and potential functions of nitrogen removal bacteria in suspended activated sludge (AS) and biofilm of a full-scale hybrid reactor were uncovered by metag...

journal_title：Applied microbiology and biotechnology

authors： Chao Y,Mao Y,Yu K,Zhang T

更新日期：2016-09-01 00:00:00

abstract：:Five types of pharmaceuticals and personal care products (PPCPs) substances were selected as pollutants in this study. The effects of the removal of these pollutants and the microbial succession process in a granular sludge membrane bioreactor (GMBR) were investigated. Results showed that wastewater containing PPCPs i...

journal_title：Applied microbiology and biotechnology

authors： Wang XC,Shen JM,Chen ZL,Zhao X,Xu H

更新日期：2016-09-01 00:00:00

abstract：:The ability of taxonomically different yeasts to synthesize pyruvic acid (PA) from glucose was studied. The study showed that many yeasts are able to produce PA from glucose under the condition of growth limitation by thiamine. This ability was found in the yeast Blastobotrys adeninivorans for the first time. The prod...

journal_title：Applied microbiology and biotechnology

authors： Kamzolova SV,Morgunov IG

更新日期：2016-09-01 00:00:00

abstract：:The knowledge of biosynthesis gene clusters, production improving methods, and bioactivity mechanisms is very important for the development of filamentous fungi metabolites. Metabolic engineering and heterologous expression methods can be applied to improve desired metabolite production, when their biosynthesis pathwa...

journal_title：Applied microbiology and biotechnology

authors： Yin Y,Cai M,Zhou X,Li Z,Zhang Y

更新日期：2016-09-01 00:00:00

abstract：:Two putative chitinase genes, lin0153 and lin1996, from the nonpathogenic bacterium Listeria innocua were expressed in Escherichia coli, and the gene products were characterized. The genes were close homologs of chitinases from the pathogenic bacterium Listeria monocytogenes, in which chitinases and chitin-binding pro...

journal_title：Applied microbiology and biotechnology

authors： Honda S,Wakita S,Sugahara Y,Kawakita M,Oyama F,Sakaguchi M

更新日期：2016-09-01 00:00:00

abstract：:A three-dimensional biofilm-electrode reactor (3DBER) was integrated with sulfur autotrophic denitrification (SAD) to improve nitrogen removal performance for wastewater reclamation. The impacts of influent carbon/nitrogen (C/N) ratio, electric current, and hydraulic retention time (HRT) were evaluated. The new proces...

journal_title：Applied microbiology and biotechnology

authors： Hao R,Meng C,Li J

更新日期：2016-08-01 00:00:00

abstract：:Globotriose (Galα1-4Galβ1-4Glc) is an important cell surface epitope that acts as the receptor for Shiga-like toxins, and it is also the core structure of Globo H and SSEA4 that are tumor-associated glycans. Hence, the enzymatic synthesis of globotriose would be necessary for the development of carbohydrate-based ther...

journal_title：Applied microbiology and biotechnology

authors： Gong W,Xu L,Gu G,Lu L,Xiao M

更新日期：2016-08-01 00:00:00

abstract：:Poly(ɛ-L-lysine) (ɛ-PL) is an unusual biopolymer composed of L-lysine connected between α-carboxyl and ɛ-amino groups. It has been used as a preservative in food and cosmetics industries, drug carrier in medicines, and gene carrier in gene therapy. Modern biotechnology has significantly improved the synthetic efficien...

journal_title：Applied microbiology and biotechnology

authors： Xu Z,Xu Z,Feng X,Xu D,Liang J,Xu H

更新日期：2016-08-01 00:00:00

abstract：:To understand the effect of daily bioaugmentation in full-scale grease interceptors (GIs), we compared the microbial communities occurring in two full-scale GIs during bioaugmented and non-bioaugmented cycles. The changes in microbial communities were determined using terminal restriction fragment length polymorphism ...

journal_title：Applied microbiology and biotechnology

authors： He X,So MJ,de Los Reyes FL 3rd

更新日期：2016-08-01 00:00:00

abstract：:The activities of seven hydrolytic enzymes (L-alanine aminopeptidase, esterase, α-and β-glucosidase, phosphomonoesterase, phosphodiesterase, sulfatase) were monitored during 1 year in parallel and serial treatment units of the biological stage of a communal wastewater treatment plant. The spatial homogeneity of enzyme...

journal_title：Applied microbiology and biotechnology

authors： Kreutz JA,Böckenhüser I,Wacht M,Fischer K

更新日期：2016-08-01 00:00:00

abstract：:The integration of physiological knowledge into process control strategies is a cornerstone for the improvement of biopharmaceutical cell culture technologies. The present contribution investigates the applicability of specific productivity as a physiological control parameter in a cell culture process producing a mon...

journal_title：Applied microbiology and biotechnology

authors： Zalai D,Hevér H,Lovász K,Molnár D,Wechselberger P,Hofer A,Párta L,Putics Á,Herwig C

更新日期：2016-08-01 00:00:00

abstract：:We collected several biofilm samples from Japanese rivers and established a reproducible multi-species biofilm model that can be analyzed in laboratories. Bacterial abundance at the generic level was highly similar between the planktonic and biofilm communities, whereas comparative metatranscriptomic analysis revealed...

journal_title：Applied microbiology and biotechnology

authors： Nakamura Y,Yamamoto N,Kino Y,Yamamoto N,Kamei S,Mori H,Kurokawa K,Nakashima N

更新日期：2016-08-01 00:00:00

abstract：:Monascus pigments (Mps) are a group of azaphilonic secondary metabolites produced by Monascus spp. via a polyketide pathway. A mutant deleted an about 30 kb region of Mps gene cluster from Monascus ruber M7 was isolated previously, which produces a high amount of a light yellow pigment. The current study revealed that...

journal_title：Applied microbiology and biotechnology

authors： Liu J,Zhou Y,Yi T,Zhao M,Xie N,Lei M,Liu Q,Shao Y,Chen F

更新日期：2016-08-01 00:00:00

abstract：:Lactobacillus plantarum MA2 was isolated from traditional Chinese Tibet kefir grains, which possess several excellent properties and functions. We previously demonstrated the antioxidant activities of this bacterium in vitro. However, the maintenance and survival of L. plantarum MA2 inside the murine intestinal tract,...

journal_title：Applied microbiology and biotechnology

authors： Tang W,Xing Z,Hu W,Li C,Wang J,Wang Y

更新日期：2016-08-01 00:00:00

abstract：:Aptamers are single-stranded nucleic acids or peptides identified from a randomized combinatorial library through specific interaction with the target of interest. Targets can be of any size, from small molecules to whole cells, attesting to the versatility of aptamers for binding a wide range of targets. Aptamers sho...

journal_title：Applied microbiology and biotechnology

authors： Gopinath SC,Lakshmipriya T,Chen Y,Arshad MK,Kerishnan JP,Ruslinda AR,Al-Douri Y,Voon CH,Hashim U

更新日期：2016-08-01 00:00:00

abstract：:Acyl-CoAs are crucial compounds involved in essential metabolic pathways such as the Krebs cycle and lipid, carbohydrate, and amino acid metabolisms, and they are also key signal molecules involved in the transcriptional regulation of lipid biosynthesis in many organisms. In this study, we took advantage of the high s...

journal_title：Applied microbiology and biotechnology

authors： Cabruja M,Lyonnet BB,Millán G,Gramajo H,Gago G

更新日期：2016-08-01 00:00:00

abstract：:The extrusion technology of blends formed by compounds with different physicochemical properties often results in new materials that present properties distinctive from its original individual constituents. Here, we report the use of melt extrusion of blends made from low-cost materials to produce a biodegradable foam...

journal_title：Applied microbiology and biotechnology

authors： Marcelino PR,Milani KM,Mali S,Santos OJ,de Oliveira AL

更新日期：2016-08-01 00:00:00